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Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study ArchiMer
Rodrigues, Sophie; Paillard, Christine; Van Dillen, Sabine; Tahrioui, Ali; Berjeaud, Jean-marc; Dufour, Alain; Bazire, Alexis.
Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A...
Tipo: Text Palavras-chave: Biofilm; Virulence; Vibrio tapetis; Transcriptome; Quorum sensing; Type VI secretion system; Brown ring disease.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00470/58140/60611.pdf
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Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components ArchiMer
Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.
Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They...
Tipo: Text Palavras-chave: Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00307/41783/42796.pdf
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